Abcam, ab227845, Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Tryptophan Hydroxylase/TPH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Recombinant fragment - Human, Recombinant fragment - Mouse samples. Cited in 9 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1311Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Product details:
ab227845 is the carrier-free version of
ab52954
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tryptophan Hydroxylase (TPH) is an enzyme that plays a critical mechanical role in the body. It is sometimes known as TPH or anti TPH. The enzyme catalyzes the conversion of tryptophan to 5-hydroxytryptophan the first and rate-limiting step in the biosynthesis of serotonin. The molecular mass of TPH is approximately 55 kDa. TPH has two isoforms TPH1 and TPH2 which are expressed in different tissues. TPH1 is mainly found in peripheral tissues such as the gut pineal gland and thymus while TPH2 is expressed in the central nervous system particularly in the raphe nuclei of the brainstem.
Biological function summary
Tryptophan hydroxylase serves an important role in serotonin production which impacts mood regulation sleep and other functions. TPH operates primarily as a homotetramer and requires biopterin oxygen and iron for its activity. Serotonin synthesized as a result acts as a neurotransmitter and hormone influencing various physiological processes. The differential expression of TPH1 and TPH2 allows for compartmentalized regulation of serotonin synthesis addressing both peripheral and central nervous system needs.
Pathways
TPH is central in the serotonin biosynthetic pathway a critical pathway for neuromodulation. It converts tryptophan into 5-hydroxytryptophan which is then decarboxylated to serotonin. TPH's function connects closely with other proteins in these pathways such as Aromatic L-amino acid decarboxylase which further processes 5-hydroxytryptophan into serotonin. This integration within the serotonin pathway highlights its importance in maintaining serotonin levels and consequently normal neurological function.
Tryptophan hydroxylase has associations with mood disorders and irritable bowel syndrome (IBS). Altered TPH activity may lead to disruptions in serotonin levels influencing development and progression of these conditions. Studies often link TPH2 with depression and anxiety due to its central neural expression and role in serotonin synthesis. Meanwhile TPH1 expressed in peripheral tissues has connections to IBS where serotonin significantly affects gut motility and sensitivity. The relationship between these disorders and TPH offers insights into therapeutic targets to modulate serotonin levels for treatment strategies.