Abcam, ab228521, Anti-MAGE3 antibody [EPR20658] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MAGE3 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20658,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody shows cross reactivity to MAGEA2 and MAGEA6 as well as weak cross reactivity to MAGEA1 and MAGEA12. It does not cross react with MAGEA4 and MAGEA8.

Product details:
ab228521 is the carrier-free version of
ab213722
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 'MAGE-A3' protein sometimes referred to simply as 'MAGE3' is part of the MAGE (Melanoma Antigen Gene) family which comprises a group of proteins known for their involvement in various cellular processes. MAGE-A3 specifically is encoded by a gene located on the X chromosome. The molecular mass of MAGE-A3 is approximately 46 kDa. This protein is typically expressed in a variety of tumor cells but is absent in most normal tissues with the exception of placental and testicular tissues. Its expression profile makes MAGE-A3 a significant focus in cancer research due to its restricted presence in normal cells and prevalent presence in transformed cells.
Biological function summary
MAGE-A3 contributes to processes related to cell cycle regulation and apoptosis. The protein is part of a complex that influences cellular stress responses and survival pathways. It exerts its biological function through interactions with other proteins potentially acting as a regulator of transcription factors involved in cell proliferation and survival. The activity and utility of MAGE-A3 in this context make it a target of interest in therapeutic research specifically for cancer vaccines and immune response strategies.
Pathways
MAGE-A3 plays a role in the regulation of the apoptotic and cell survival pathways. It is notably involved in pathways such as the p53 pathway essential for modulating cellular responses to stress and DNA damage. MAGE-A3 interacts with proteins like p53 and other regulators involved in the cell cycle and apoptosis thereby affecting cellular decisions to repair damage or undergo programmed cell death. Its role in these pathways highlights its importance in maintaining the balance between cell survival and death highlighting its potential target for therapeutic intervention.
MAGE-A3 is closely associated with cancers such as melanoma and non-small cell lung cancer. Its expression in these malignancies makes it a prime candidate for cancer-specific targeted therapies including cancer vaccines. MAGE-A3 is connected with proteins that regulate immune evasion and tumor growth making its modulation an attractive strategy in immunotherapy. Understanding the interaction of MAGE-A3 with these proteins provides valuable insights necessary for developing precise diagnostic and treatment methods for these cancers.