Abcam, ab228569, TMRM Assay Kit (Mitochondrial Membrane Potential)

Size: 1Kit
TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is a complete kit for TMRM staining of cell cultures. Readout by flow cytometry, fluorescence plate reader, or fluorescence microscopy (Ex/Em 548/573 nm). Contains TMRM reagent, buffer and control.
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Microplate reader, Fluor. microscope, Flow cyt.

Product details:
TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.
Related assay kits
TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
TMRE-Mitochondrial Membrane Potential Assay Kit
ab113852
is our most popular assay kit for mitochondrial membrane potential analysis.
TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically. This kit provides enough reagents to analyze 5 x 96 wells (500 samples).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols