Abcam, ab229074, Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CEACAM5 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20721,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WB, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab229074 cross-reacts with CEACAM 1, 6 and 8. There are other CEACAM family members but we only examined cross-reactivity with those having >55% homology with CEACAM5 following a BLAST search with the immunogen.

Product details:
ab229074 is the carrier-free version of
ab207718
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CEACAM5 also known as CEA or CD66e is part of the carcinoembryonic antigen family. It plays a role as a cell adhesion molecule facilitating intercellular binding. CEACAM5 is a glycoprotein with a molecular mass of approximately 180 kDa. You find CEACAM5 expression in normal epithelial tissues such as the colon stomach and tongue but it shows increased levels in various carcinoma cells. It is also identified as CEACAM6 and both of these linked proteins function as cell surface glycoproteins involved in cellular interactions.
Biological function summary
CEACAM5 proteins influence cell signaling and intercellular communication. They participate in immune response modulation and function as part of larger protein complexes. CEACAM5 acts as an anchor point for various cellular processes by associating with CEACAM1 and CEACAM6. By regulating cell adhesion CEACAM5 helps in maintaining normal cellular architecture and function within tissue environments.
Pathways
CEACAM5 is integral to pathways involving cell adhesion and signaling. It participates in immune system responses and the regulation of cell proliferation pathways including the MEK/ERK signaling cascade. CEACAM5 proteins interact with others like CEACAM1 sharing roles in cell signaling and communication networks that control adhesion dynamics and growth factor responses.
Excessive CEACAM5 expression links to colorectal and gastric cancers making it a useful cancer biomarker. CEACAM5-positive tumors often correlate with disease progression which highlights their potential as therapeutic targets. These proteins also interact with CD66b markers which are associated with neutrophil activation in inflammatory responses. Such associations with CEACAM1 and CEACAM6 in carcinogenic pathways further underline CEACAM5's relevance in cancer diagnostics and treatment strategies.