Abcam, ab229102, Anti-ZNF598 antibody

Size: 100µL
Rabbit Polyclonal ZNF598 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF598.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ZNF598. The exact immunogen used to generate this antibody is proprietary information.Q86UK7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZNF598 also known as Zinc Finger Protein 598 functions as an E3 ubiquitin ligase involved in ribosome-associated quality control. It has a molecular mass of approximately 98 kDa. This protein contains multiple zinc finger domains that mediate its binding to substrates. ZNF598 localizes predominantly to the cytoplasm specifically associating with ribosomes. Expression occurs in various tissues with higher levels observed in actively dividing cells.
Biological function summary
ZNF598 regulates translation quality control by recognizing and ubiquitinating stalled 80S ribosomal complexes marking them for degradation. This process is part of the Ribosome-Associated Quality Control (RQC) complex. It ensures that defective mRNAs and nascent peptide chains do not accumulate maintaining the fidelity of protein synthesis. Through interaction with other components of the RQC complex ZNF598 prevents the persistence of translation errors that could be harmful to the cell.
Pathways
ZNF598 plays a critical role in the Nonsense Mediated Decay (NMD) pathway and the Integrated Stress Response (ISR). It collaborates with other proteins such as GADD34 and NEMF to manage ribosomal stalling and subsequent protein degradation. In the NMD pathway ZNF598 ensures the rapid degradation of transcripts with premature stop codons. In the ISR ZNF598's activity assists in modulating translation under conditions of cellular stress.
ZNF598's malfunction associates with neurodegenerative conditions and cancer. Mutations affecting its function can lead to translational errors contributing to the accumulation of toxic protein aggregates seen in disorders like amyotrophic lateral sclerosis (ALS). Additionally overexpression or aberrant activity of ZNF598 links to tumorigenesis as it can alter translational homeostasis. During these disease states interactions with proteins such as ATXN2 and PIK3CA emphasize the critical nature of maintaining translational integrity.