Abcam, ab229278, Salmon Sperm ssDNA

Size: 5mg
This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods.
Key facts
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 7.6Constituents: 0.16% Tris HCl, 0.03% EDTA

Product details:
This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency.
Background
: Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from salmon testes DNA by mechanical shearing and heat denaturation to an average size of 100 to 200 base pairs. To reverse any renaturation occurring during storage, this material should be briefly boiled and rapidly chilled prior to use. >75% native nucleic acid.
Purity/Specifity
: A260/A280: 1.85 % Hyperchromocity: 30.5.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle