Abcam, ab229351, Anti-TRUB1 antibody

Size: 100µL
Rabbit Polyclonal TRUB1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRUB1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TRUB1. The exact immunogen used to generate this antibody is proprietary information.Q8WWH5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRUB1 also known as TruB pseudouridine synthase family member 1 is an enzyme with a molecular mass of approximately 58 kDa. It is primarily involved in the pseudouridylation of RNA molecules which is a process where uridine residues in RNA are converted to pseudouridine. This protein shows expression in various tissues with notable presence in the nucleolus of eukaryotic cells where it performs its functions on RNA. TRUB1 has homologs across different species indicating a conserved role in RNA processing.
Biological function summary
TRUB1 interacts with components of the ribonucleoprotein complex playing a role in the maturation and structural modification of ribosomal RNA. This modification helps improve the stability and functionality of RNA molecules enhancing their ability to participate in translation. TRUB1 achieves its function by recognizing specific sequences in RNA substrate and performing the enzymatic conversion to pseudouridine thereby impacting RNA stability and translation fidelity across various cell types.
Pathways
RNA modifications where TRUB1 is involved play a critical role in cellular translation mechanisms. It participates in pathways that include the ribosome biogenesis pathway and RNA modification pathways. Other proteins that are part of these pathways such as dyskerin and fibrillarin share similar sites of action in the nucleolus and work collaboratively to support the proper formation of ribosomal RNA allowing TRUB1 to be intricately linked with these processes.
TRUB1 has been implicated in ribosomopathies which are disorders caused by defects in ribosome function and certain cancers where alteration in RNA processing pathways is observed. Its association with dyskerin another pseudouridine synthase highlights a potential connection to diseases like dyskeratosis congenita. These diseases manifest through defects in ribosome assembly and function where TRUB1's role in pseudouridylation may directly influence cellular protein synthesis contributing to the disease pathology.