Abcam, ab229384, Human IL-1 beta ELISA Kit, Fluorescent

Size: 1 x 96Tests
Human IL-1 beta ELISA Kit, Fluorescent is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 beta , Fluorescent in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Fluorescent,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.6 pg/mL,
Range:3.5 - 3600 pg/mL,
Assay time:2h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
IL-1 beta
in vitro
CatchPoint
ELISA kit is designed for the quantitative measurement of IL-1 beta protein in human serum, plasma and cell culture supernatants.
This CatchPoint ELISA kit has been
optimized for Molecular Devices Microplate Readers
. Click
for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint ELISA Kits is available with all the protocol and analysis settings at
www.softmaxpro.org
The CatchPoint
ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C