Abcam, ab229497, Anti-NOL5A antibody - C-terminal

Size: 100µL
Rabbit Polyclonal NOL5A antibody. C-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NOP56.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NOP56. The exact immunogen used to generate this antibody is proprietary information.O00567

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOL5A also known as Nucleolar Protein 5A or hNop56 functions as a nucleolar protein involved in ribosome biogenesis. It is characterized by a mass of approximately 63 kDa. NOL5A exhibits high expression within the nucleolus of cells where it participates in the assembly of ribosomal subunits. Specifically NOL5A plays a role in the modification and maturation of pre-ribosomal RNA. Its expression is observed in rapidly dividing cells highlighting its importance in cellular growth and proliferation.
Biological function summary
Ribosome biogenesis depends on several proteins and NOL5A serves as a core component in the small nucleolar ribonucleoprotein (snoRNP) complex. This complex is responsible for the processing and chemical modification of small nucleolar RNAs (snoRNAs) which are essential for the production of functional ribosomes. NOL5A helps facilitate the cleavage and assembly steps necessary for pre-rRNA to mature into functional rRNAn important for effective protein synthesis within the cell. Its activity is important to maintaining proper ribosome function ensuring efficient protein translation.
Pathways
NOL5A acts within the rRNA processing pathway and is also linked to the mTOR signaling pathway. In the rRNA processing pathway NOL5A's role in the nucleoplasm involves cooperation with other nucleolar proteins like fibrillarin and Nop58 to orchestrate rRNA processing. In the mTOR pathway the regulation of ribosome production connects NOL5A to protein synthesis pathways impacting cell growth and proliferation. mTOR signaling influences many cellular processes and NOL5A’s function in ribosome biogenesis links it to these critical growth and survival pathways.
NOL5A has connections with cancer and ribosomopathy. Altered expression of NOL5A has implications in various cancers as its role in ribosome production can drive aberrant cell proliferation. Additionally proper function of NOL5A is essential in preventing ribosomopathies disorders characterized by defects in ribosome biogenesis. These disorders often result in bone marrow failure or craniofacial abnormalities. Studies investigating NOL5A's interactions with proteins such as NPM1 show its involvement in nucleolar stress responses which can influence cancer progression and response to therapy.