Abcam, ab229564, Anti-TTF1/Transcription termination factor 1 antibody

Size: 100µL
Rabbit Polyclonal TTF1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TTF1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TTF1. The exact immunogen used to generate this antibody is proprietary information.Q15361

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TTF1 also known as Transcription Termination Factor 1 plays a critical role in transcription termination of ribosomal RNA genes. This protein with a molecular mass of approximately 90 kDa specifically binds to terminator sequences facilitating the release of paused RNA polymerase I. TTF1 is widely expressed in eukaryotic cells particularly in tissues with high rates of ribosomal RNA synthesis like the nucleolus within the nucleus. Its presence is essential for the proper termination and recycling of the transcription machinery enhancing the efficiency of ribosomal RNA production.
Biological function summary
TTF1 influences the regulation and expression of ribosomal RNA by forming a complex with other factors to ensure successful transcription termination and reinitiation. It interacts with co-factors such as Nucleolin and facilitates the looping of DNA which is a necessary component of efficient termination. This function supports cellular growth and proliferation by maintaining high levels of ribosome synthesis which is essential for protein translation.
Pathways
TTF1 is significantly associated with the nucleolar transcription termination pathway and is integral to the modulation of ribosomal RNA synthesis. It works alongside proteins such as RNA Polymerase I and Transcription Initiation Factor SL1 forming a relationship that ensures efficient transcriptional processes. TTF1 helps maintain the balance between transcription initiation elongation and termination important for the homeostasis of cell proliferation and growth pathways.
TTF1 dysregulation relates to oncogenesis and certain ribosomopathies where abnormal ribosome function can occur. Overexpression or mutation of TTF1 can lead to increased ribosome production contributing to the unrestrained growth seen in certain cancers. It collaborates with proteins such as c-Myc which is known for promoting cell proliferation and is frequently upregulated in cancerous cells. Understanding TTF1's role offers insights into potential therapeutic targets for treating these conditions.