Abcam, ab229856, Anti-TGF beta 1 antibody [EPR21143] - BSA and Azide free

Size: 100µg / 1mg
Anti-TGF beta 1 antibody [EPR21143] - BSA and Azide free (ab229856) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting TGF beta 1 in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21143,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:For testing samples with low expression level of TGF beta 1, we recommend ab179695 which could give stronger signal. Loading larger amount of lysate or lower antibody dilution would also help.

Product details:
What is this antibody validated in?
Anti-TGF beta 1 antibody [EPR21143] - BSA and Azide free (ab229856) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of TGF beta 1?
Anti-TGF beta 1 [EPR21143] - BSA and Azide free (ab229856) specifically detects a band for TGF beta 1 (UniProt: P04202) at a molecular weight of 44kDa.
Specificity confirmed
The specificity of Anti-TGF beta 1 antibody [EPR21143] - BSA and Azide free (ab229856) has been confirmed by Western blot testing in TGFB1 Knockout HeLa cell line.
Other related products
We have a range of other formats of antibody clone [EPR21143] also available for your convenience:
ab215715
, Carrier free - ab229856, Alexa Fluor® 647 -
ab281316
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze