Abcam, ab229912, Anti-PERK antibody [EPR19876-294]

Size: 20µL / 100µL / 1mL
Anti-PERK antibody [EPR19876-294] (ab229912) is a rabbit monoclonal antibody detecting PERK in Western Blot . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19876-294,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-PERK antibody [EPR19876-294] (ab229912) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of PERK?
Anti-PERK [EPR19876-294] (ab229912) specifically detects a band for PERK (UniProt: Q9NZJ5) at a molecular weight of 125kDa.
Trusted by the scientific community
Anti-PERK [EPR19876-294] (ab229912) was first used in a scientific publication in 2019 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-PERK [EPR19876-294] (ab229912) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-PERK antibody [EPR19876-294] (ab229912) has been confirmed by Western blot testing in EIF2AK3 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR19876-294] also available for your convenience: ab229912, Carrier free -
ab254249
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PERK also known as EIF2AK3 is a protein kinase involved in the unfolded protein response (UPR). Researchers commonly identify it by its phosphorylation state in western blots recognizable as phospho-PERK or p-PERK. The molecular weight of PERK is approximately 125 kDa. It is predominantly expressed in the endoplasmic reticulum (ER) of cells acting as a sensor for misfolded proteins. PERK acts mechanistically by phosphorylating the translation initiation factor eIF2α reducing overall protein synthesis and alleviating ER stress.
Biological function summary
PERK plays an important role in maintaining cellular homeostasis during stress conditions. It forms part of a larger complex that regulates the adaptive response to ER stress. Upon activation PERK arrests general protein translation while allowing for selective translation of stress response proteins. This mechanism prevents the accumulation of unfolded proteins and assists in the survival of cells under adverse conditions. PERK also contributes to the regulation of oxidative stress and cell apoptosis adding further layers to its biological significance.
Pathways
PERK's activity intersects with the integrated stress response and UPR pathways. It shares a close functional relationship with proteins like ATF4 which is synthesized upon PERK activation and is important for the transcription of genes related to stress adaptation. The UPR pathway coordinates with the IRE1 and ATF6 pathways forming an important network for managing ER stress. These pathways collectively ensure cellular resilience adjusting metabolic demands and promoting cellular recovery.
PERK's malfunction can contribute to conditions such as diabetes and neurodegenerative diseases like Alzheimer's. In diabetes improper regulation of PERK affects insulin synthesis and secretion leading to pancreatic Β-cell dysfunction. Alzheimer's disease shows links with heightened ER stress and dysregulation of the PERK-eIF2α signaling pathway potentially contributing to neuronal death. PERK dysfunction can also associate with other proteins such as CHOP which mediates apoptosis during prolonged ER stress exacerbating these conditions.