Abcam, ab23, Anti-CD45RO antibody [UCHL1]

Size: 100µg
Anti-CD45RO antibody [UCH-L1] (ab23) is a mouse monoclonal antibody detecting CD45RO in Western Blot, Flow Cytometry, IHC-P . Suitable for Human . - Over 20 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:UCHL1,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-CD45RO antibody [UCH-L1] (ab23) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of CD45RO?
Anti-CD45RO [UCH-L1] (ab23) specifically detects a band for CD45RO (UniProt: P08575) at a molecular weight of 131kDa.
Trusted by the scientific community
Anti-CD45RO [UCH-L1] (ab23) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD45RO also known as leukocyte common antigen 2 is an important isoform of the protein tyrosine phosphatase receptor type C (PTPRC). This protein has a molecular mass of about 180 kDa. Researchers find CD45RO expressed mostly on memory T cells a subset of T lymphocytes. These cells play an essential role in the immune system by remembering past infections or vaccinations. CD45RO functions mechanistically by modulating T cell receptor (TCR) signaling which is important for antibody production.
Biological function summary
This isoform plays an important role in immune responses by supporting the activation and differentiation of T cells. It is not part of a larger protein complex itself but interacts with other immune cell surface proteins for effective signaling. CD45RO is particularly important in the modulation of signal transduction through its phosphatase activity which removes phosphate groups from specific tyrosine residues on proteins influencing T cell sensitivity and response to antigens.
Pathways
CD45RO influences the JAK/STAT pathway a critical signaling pathway for cytokine receptor signaling in immune cells. It also plays a role in the TCR signaling pathway which is responsible for T cell activation. Within these pathways CD45RO acts in conjunction with other proteins like Lck and ZAP-70 that are also integral parts of T cell activation and proliferation processes essential for adaptive immune responses.
CD45RO is associated with autoimmune disorders such as rheumatoid arthritis where memory T cells play a role in sustaining inflammation. CD45RO also relates to chronic hepatitis B infection as it affects the disease progression through memory T cell-mediated immune responses. In these contexts CD45RO's interaction with other proteins like HLA-DR which is another immune-related surface protein becomes significant as it helps modulate immune responses that either suppress or exacerbate the disease symptoms.