Abcam, ab230692, Anti-Rhodopsin antibody [B630]

Size: 50µL
Mouse Monoclonal Rhodopsin antibody. Suitable for IHC-P, WB and reacts with Pig, Human, Cow samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow Rhodopsin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:B630,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Cow, Human, Pig,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Cow Rhodopsin.P02699

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Protein G purified from tissue culture supernatant., Storage buffer-Preservative: 0.07% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rhodopsin also known as visual purple is a protein found in the photoreceptor cells of the retina. It has a mass of approximately 39 kDa. This protein acts as a light-sensitive receptor playing an essential role in the function of rod cells. Rhodopsin is expressed predominantly in the rod outer segment where it transduces light signals. The crystallized form of this receptor referred to as PDB ID: 1D4 has helped researchers study its structure in detail.
Biological function summary
Rhodopsin enables night vision by absorbing photons and activating a G-protein-coupled receptor (GPCR) cascade. Rhodopsin is an integral part of a protein complex within the phototransduction pathway where it works closely with transducin to amplify the visual signal. It initiates the conversion of the photon into an electrical signal. Rhodopsin's role is triggering a structural change in response to light converting from its inactive form to a signaling state.
Pathways
Rhodopsin is deeply involved in the phototransduction and retinoid cycle pathways. Once it absorbs light the transducin it binds causes the activation of a cascade that ultimately results in hyperpolarization of the rod cells. This network of interactions also involves arrestin and recoverin which work together to regulate rhodopsin activity and signal termination. The process ensures precise response and recovery of the visual signal in low-light conditions.
Rhodopsin mutations and malfunctions connect to retinitis pigmentosa and congenital stationary night blindness. Mutations can lead to improper folding or misregulation of the protein impacting its function. Visual cycle proteins like RPE65 are often affected alongside rhodopsin in retinitis pigmentosa. These associations stress rhodopsin's importance in maintaining healthy retinal function and its link to eye diseases.