Abcam, ab232466, Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ARTS1/ERAP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6069,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Product details:
ab232466 is the carrier-free version of
ab124669
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARTS1 also known as ERAP1 (Endoplasmic Reticulum Aminopeptidase 1) is an aminopeptidase enzyme with a molecular mass of about 110 kDa. This protein is located in the endoplasmic reticulum and plays a significant mechanical role in trimming peptides for presentation by MHC class I molecules. Its expression is highly detectable in cells with robust antigen-presenting capabilities such as dendritic cells and macrophages. Additionally ARTS1/ERAP1 exists in other tissues where immune surveillance is essential.
Biological function summary
The function of ARTS1/ERAP1 involves the processing of proteolytic fragments preparing them for optimal binding to MHC class I molecules. This enzyme participates in a complex alongside ERAP2 another aminopeptidase working cooperatively to adjust peptide lengths to the correct size necessary for MHC class I presentation. Through this role ARTS1/ERAP1 influences the immune system's ability to detect and respond to infected or neoplastic cells by ensuring that antigenic peptides are properly presented.
Pathways
ARTS1/ERAP1 is a critical component of the antigen processing and presentation pathway. This pathway is essential for the presentation of endogenous antigens to cytotoxic T lymphocytes therefore playing a role in adaptive immunity. Related proteins include MHC class I molecules which present the processed peptides and TAP (Transporter associated with Antigen Processing) which transports peptides into the endoplasmic reticulum for further trimming by ARTS1/ERAP1.
Research links ARTS1/ERAP1 to ankylosing spondylitis and other autoimmune diseases. Variations in the ERAP1 gene may affect peptide trimming efficiency altering the peptide repertoire presented by MHC class I molecules and influencing autoimmune activation. The link to another protein HLA-B27 is notable in ankylosing spondylitis as this MHC class I allele frequently associates with the disease highlighting a complex interaction between ARTS1/ERAP1 enzymatic function and autoimmune susceptibility.