Abcam, ab232798, Anti-PDHX antibody

Size: 100µg
Rabbit Polyclonal PDHX antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDHX aa 200-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Pig,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PDHX aa 200-500. The exact immunogen used to generate this antibody is proprietary information.O00330

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDHX also known as pyruvate dehydrogenase protein X is a part of the pyruvate dehydrogenase complex. It has a molecular mass of about 54 kDa. PDHX is expressed mostly in tissues with high energy demands such as the heart and skeletal muscle. The protein has a critical role in linking glycolysis and the Krebs cycle by transferring the lipoamide group in the pyruvate dehydrogenase complex.
Biological function summary
PDHX functions as a component of the multi-enzyme pyruvate dehydrogenase complex (PDC). This complex is essential for the oxidative decarboxylation of pyruvate to acetyl-CoA within the mitochondria. PDHX coordinates with other enzymes such as E1 E2 and E3 carrying out its function by acting as an anchor to stabilize the PDC enhancing the efficiency of the catalytic process.
Pathways
PDHX participates in important metabolic pathways like glycolysis and the Krebs cycle. It relates closely with proteins such as E1 (pyruvate dehydrogenase) and E2 (dihydrolipoamide acetyltransferase) due to its role in facilitating the transition of energy substrates into the cycle. Its activity helps in the regulation of energy production within cells and impacts processes that depend on efficient energy conversion.
Mutations or deficiencies in PDHX have connections with pyruvate dehydrogenase deficiency and metabolic disorders like Leigh syndrome. These conditions affect energy metabolism and can lead to severe neurological problems. The malfunction of PDHX impacts the pyruvate dehydrogenase complex as a whole leading to an increase of pyruvate levels and a decrease in ATP production linking it also with other involved proteins like E1 and E2.