Abcam, ab232989, Anti-Glycine decarboxylase antibody

Size: 100µg
Rabbit Polyclonal Glycine decarboxylase antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLDC aa 50-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Pig,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GLDC aa 50-300. The exact immunogen used to generate this antibody is proprietary information.P23378

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycine decarboxylase also known as P-protein catalyzes the decarboxylation of glycine. This reaction releases carbon dioxide and transfers the remaining methylamine to tetrahydrofolate. Glycine decarboxylase is an essential component of the glycine cleavage system which consists of four proteins: P H T and L-proteins. The molecular mass of glycine decarboxylase is approximately 1000 kDa. This protein is mainly expressed in the mitochondria of liver and kidney cells where it plays an important role in energy metabolism.
Biological function summary
Glycine decarboxylase functions in cellular metabolism and photorespiration. It is part of the glycine cleavage system complex which is responsible for breaking down glycine into CO2 NH3 and a methylene group. The methylene group then enters the folate pool and contributes to various one-carbon metabolism pathways. This process helps regulate cellular levels of glycine and serine both important for nucleotide and protein synthesis as well as maintaining the balance of oxidative stress within cells.
Pathways
Glycine decarboxylase plays an important role in the folate and photorespiratory pathways. In the folate pathway interactions with serine hydroxymethyltransferase allow for the conversion of serine to glycine and the transfer of one-carbon units needed for nucleic acid synthesis. In the photorespiratory pathway its activity helps recycle 2-phosphoglycolate in plant mitochondria minimizing energy loss. The protein dihydrolipoamide dehydrogenase part of the same enzyme complex assists in maintaining the cycle of oxidation and reduction necessary for these functions.
Glycine decarboxylase dysfunction associates with nonketotic hyperglycinemia and certain liver diseases. In nonketotic hyperglycinemia defective glycine cleavage leads to excess glycine accumulation causing neurological symptoms such as seizures and developmental delays. Liver diseases linked to abnormal glycine metabolism can arise from impaired liver enzyme activities including those involving glycine decarboxylase. Another related protein AMT (aminomethyltransferase) also contributes to such conditions when mutations affect the glycine cleavage system.