Abcam, ab233486, Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)

Size: 1 x 48Tests / 1 x 96Tests
Global DNA Methylation Assay Kit (5-Methyl Cytosine) ab233486 contains all reagents necessary for the quantification of global DNA methylation. Readout on any colorimetric (450 nm) plate reader. - Detection limit as low as 0.05% methylated DNA from 100 ng of input DNA - High specific for 5-Methylcytosine with no cross-reactivity to unmethylated cytosine or hydroxymethylcytosine within the indicated concentration range of the sample DNA - Universal positive and negative controls are included for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA
Key facts
Detection method:Colorimetric,
Sample types:DNA,
Assay type:Quantitative,
Assay time:2h,
Assay Platform:Microplate reader

Product details:
How the assay works
In the global DNA methylation assay ab233486, DNA is bound to strip-wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer. The percentage of methylated DNA is proportional to the OD intensity measured.
Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) protocol summary:
- Bind DNA to assay wells
- Wash wells, then add detection complex solution.
- Wash and add color developer solution.
- Measure absorbance at 450 nm
How other researchers are using
Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) has been used in a variety of sample type including:
- Human Tumor and control peritoneal tissues 1
- Human abdominal skin samples 2
- Mouse brain tissues 3
References:
1 - Reamon-Buettner S et al. 2024; 2 - Ghaffarinia A et al. 2023; 3 - Spandole-Dinu S et al. 2023.
Related and recommended products
See an alternative DNA Methylation Assay Kit for the quantification of m6A in DNA:
- m6A DNA Methylation Assay Kit (Colorimetric)
ab233488

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols