Abcam, ab233561, Anti-PRKAG3 antibody [1C5D10]

Size: 100µg
Mouse Monoclonal PRKAG3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PRKAG3 aa 1-200.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1C5D10,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PRKAG3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.Q9UGI9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRKAG3 also known as AMP-activated protein kinase subunit gamma-3 is a regulatory subunit of the AMP-activated protein kinase (AMPK) complex. The gamma-3 subunit contributes to the full AMPK function particularly its sensitivity to energy status changes within cells. This subunit weighs approximately 38.1 kDa and is mainly expressed in skeletal muscle tissue. It plays an important role in cellular energy homeostasis by responding to depletion in intracellular ATP levels.
Biological function summary
PRKAG3 interacts with AMP and ADP which activates AMPK when energy levels are low. It forms a complex with two other subunits the alpha (catalytic) and beta (regulatory) subunits to become fully active. Once activated AMPK executes various cellular functions including the regulation of glucose uptake and fatty acid oxidation primarily within muscle tissues which is essential for maintaining energy balance.
Pathways
PRKAG3 takes part in the AMPK signaling pathway a central regulator of metabolic pathways that maintain cellular energy balance. It connects to glucose and lipid metabolic processes interacting with proteins like ACC (acetyl-CoA carboxylase) for fatty acid synthesis inhibition and GLUT4 (glucose transporter type 4) for glucose uptake enhancement. Through these interactions PRKAG3 influences critical metabolic adaptations to energy stress.
Mutations or dysregulation in PRKAG3 have links with glycogen storage disease type V (McArdle disease) and insulin resistance. In particular disorders in glycemic control connect this protein to the regulation of glucose metabolism. PRKAG3 mutations can affect the typical glycogen synthesis and breakdown in skeletal muscle impacting energy availability during exercise. Additionally its activity has associations with insulin-signaling networks in conjunction with proteins like insulin receptor substrate-1 (IRS-1) impacting glucose homeostasis and insulin sensitivity.