Abcam, ab233621, Mouse GFAP ELISA Kit

Size: 1 x 96Tests
Mouse GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GFAP with a sensitivity of 8.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.7 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse GFAP ELISA Kit ab233621 is a rapid single-wash 90-min ELISA kit to measure Mouse GFAP in tissue extracts. This SimpleStep ELISA® sensitivity is 8.7 pg/mL.
How the assay works
Mouse GFAP SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse GFAP protein in tissue extract samples only.
Mouse GFAP ELISA Kit ab233621 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse GFAP ELISA Kit ab233621
Mouse GFAP ELISA Kit ab233621 has been used to develop a nanosensor to identify mild traumatic brain injury
, to test resolvin D2 as a therapy for neuropathic pain and bone cancer pain
, and to study clearance of microglial debris for CNS homeostasis
References:
Ester J Kwon et al. 2023, PMID: 37873031,
Xiaopeng Wang et al. 2023, PMID: 36672133,
Bo Peng, et al. 2022, PMID: 36280666
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Mouse GFAP Antibody Pair - BSA and Azide free
ab244094
- Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture)
ab242692
- Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector)
ab242937
Related and recommended products
Mouse GFAP ELISA Kit ab233621 is commonly used to measure neuroinflammatory diseases such as Alzheimer's Disease and traumatic brain injury.
Other ELISA kits to measure neuroinflammation include:
- Mouse IL-6 ELISA kit
ab222503
- Mouse TNF alpha ELISA kit
ab208348
- Mouse IL-1 beta ELISA Kit
ab197742
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C