Abcam, ab234054, Phagocytosis Assay Kit (Red Zymosan)

Size: 100Test
Phagocytosis Assay Kit ab234054 uses Zymosan particles, that are fluorescently labeled with a red dye, to detect and quantify in vitro phagocytosis by fluorescent microscope, fluorometric plate reader or flow cytometry. - Ex/Em 540/570 nm - Validated as macrophage phagocytosis assay. - includes labeled Zymosan particles and assay buffer. Also includes quenching solution for readout on microscope / plate reader.
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay Platform:Microplate reader, Fluor. microscope, Flow cyt.

Product details:
Phagocytosis Assay Kit ab234054 uses Zymosan particles which have been pre-labeled with a red dye.
The assay can be used for the rapid and accurate detection and quantification of
in vitro
phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. It provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).
The Zymosan particles used in the phagocytosis assay protocol are labeled for fluorescence at Ex/Em 540/570 nm.
Phagocytosis assay protocol summary
- add Red Zymosan reagent to cells, and incubate for 2-3 hours
- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times
- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution
- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader
Related phagocytosis assays
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ab235900
Phagocytosis Assay Kit (Red E. coli)
ab235901
Phagocytosis Assay Kit (Green Zymosan)
ab234053
Phagocytosis Assay Kit (Zymosan, Colorimetric)
ab211156
Other notes
This product was previously called K398 Biovision EZCell™ Phagocytosis Assay Kit (Red Zymosan). Biovision was acquired by Abcam in 2021.
In mammals, phagocytosis by phagocytes (e.g., macrophages, dendritic cells, and neutrophils) is essential for a variety of biological events. Phagocytosis comprises a series of events, starting with the binding and recognition of particles by cell surface receptors, followed by the formation of actin-rich membrane extensions around the particle.
Zymosan (Saccharomyces cerevisiae) is prepared from yeast cell wall and consists of protein-carbohydrate complexes. Zymosan is a commonly used pathogen in phagocytosis assays.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols