Abcam, ab234745, Anti-ASH1L antibody

Size: 100µg / 100µL
Rabbit Polyclonal ASH1L antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASH1L aa 1900-2200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ASH1L aa 1900-2200. The exact immunogen used to generate this antibody is proprietary information.Q9NR48

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ASH1L also known as Absent Small or Homeotic-Like 1 is a protein-coding gene that functions as a histone lysine methyltransferase. This enzyme has a large mass of around 296 kDa and is mainly expressed in the human brain and the testes. It is located in the euchromatic region and participates in depositing methyl groups on lysine residues of histones which influences chromatin structure and gene expression. Due to its ability to modify chromatin ASH1L plays a significant role in regulating transcription.
Biological function summary
ASH1L contributes to various processes involving transcriptional regulation and chromatin modification. This protein functions as part of the Set1/Ash1 complex where it influences gene expression by specifically methylating histone H3 at lysine 36 (H3K36). The methylation of this residue is associated with active transcription and is important for the proper regulation of genes involved in development and differentiation. ASH1L-mediated modifications thereby facilitate the global maintenance of the active chromatin state essential for cellular processes.
Pathways
ASH1L is engaged in pathways related to histone modification and transcriptional regulation. It functions alongside proteins in the Wnt signaling pathway which plays a vital role in regulating tissue-specific transcription programs. ASH1L can alternatively interact with other histone methyltransferases like the NSD family which share similar functions in chromatin dynamics and are involved in transcription elongation and chromatin remodeling.
ASH1L has been associated with developmental disorders and certain cancers. Its function in epigenetic regulation makes it significant in neurodevelopmental disorders where disruptions in its expression or function can lead to conditions such as autism spectrum disorders. Additionally ASH1L's misregulation has been linked to leukemia where it interacts with mixed-lineage leukemia (MLL) proteins due to its role in regulating gene expression involved in cell proliferation.