Abcam, ab234901, Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TRP2/DCT antibody. Carrier free. Suitable for IP, WB, IHC-P, mIHC and reacts with Mouse, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21986,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:IHC-P, mIHC, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab234901 is the carrier-free version of
ab221144
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRP2 also known as DCT or dopachrome tautomerase is a protein involved in melanin synthesis and is part of the tyrosinase-related protein family. It has a molecular weight of approximately 60 kDa. TRP2/DCT is mainly expressed in melanocytes which are cells responsible for pigment production. The protein functions in the melanin biosynthetic pathway converting dopachrome into DHICA contributing to the eumelanin formation which impacts skin and hair color.
Biological function summary
TRP2/DCT participates in pigmentation and photoprotection processes. It functions in tandem with other enzymes like tyrosinase and TRP1 to form a multiprotein complex important for melanin synthesis. The coordination of these proteins ensures the effective production and regulation of melanin which absorbs ultraviolet radiation and reduces DNA damage in skin cells.
Pathways
TRP2/DCT plays a role in the melanin biosynthetic pathway and influences the Wnt signaling pathway. The melanin pathway involves enzymes such as tyrosinase which initiates the conversion of tyrosine to L-DOPA and eventually to melanin pigments. The Wnt signaling pathway critical for cellular processes like proliferation and differentiation interacts with melanogenesis through proteins like beta-catenin influencing TRP2/DCT expression and activity.
TRP2/DCT has significant associations with melanoma and vitiligo. In melanoma altered expression of TRP2/DCT can impact tumor cell proliferation and survival often interacting with proteins like MITF an important regulator of melanocyte development. In vitiligo changes in TRP2/DCT expression can disrupt melanin production leading to depigmented patches on the skin. Understanding these interactions and expressions aids in potential therapeutic approaches for these skin conditions.