Abcam, ab235385, Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal cAMP Protein Kinase Catalytic subunit antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2102Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, ICC/IF, WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen used for this product shares 92% homology with PKA C-beta and PKA C-gamma. Cross-reactivity with these proteins have not been confirmed experimentally.

Product details:
ab235385 is the carrier-free version of
ab76238
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cAMP Protein Kinase Catalytic subunit also known as protein kinase A (PKA) catalytic subunit plays an important role as an enzyme that phosphorylates various target proteins. This is an important component of the protein kinase A signaling pathway. The subunit has an approximate mass of 40 kDa and is widely expressed in multiple tissues across the body allowing it to affect numerous physiological functions. As a catalytic subunit of cAMP-dependent protein kinase it forms part of the larger enzyme complex that is activated by the binding of cAMP.
Biological function summary
The catalytic subunit of protein kinase A modulates cellular processes by transferring a phosphate group from ATP to specific serine/threonine residues on substrates. It is part of a holoenzyme complex composed of two regulatory and two catalytic subunits; when cAMP binds to the regulatory subunits it releases the catalytic subunits allowing them to execute their activity. This release and activation result in the regulation of glycogen sugar and lipid metabolism among others.
Pathways
The cAMP Protein Kinase integrates deeply into the cAMP signaling and MAPK/ERK pathways. These pathways regulate various processes like cell growth differentiation and apoptosis. The catalytic subunit acts downstream of cAMP generation by adenylyl cyclase interacting with other proteins such as CREB (cAMP response element-binding protein) which directly affects transcription and gene expression.
The cAMP Protein Kinase catalytic subunit has been linked to conditions including Carney complex and Cushing’s syndrome. Mutations affecting this subunit can lead to dysregulated cAMP signaling impacting proteins like regulatory subunit PRKAR1A that are important in controlling PKA activity. Conditions such as these often arise from aberrations in cAMP signaling pathways reflecting the extensive influence of the cAMP Protein Kinase on homeostasis and disease.