Abcam, ab236037, Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Lhx2/LH2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20449,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IHC-Fr, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab236037 is the carrier-free version of
ab184337
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Lhx2 protein also known as LH2 is a member of the LIM homeobox family of transcription factors. It has a molecular mass of approximately 45 kDa. Known for its dual LIM domains and a homeodomain Lhx2 directly binds DNA and regulates gene expression. This protein is expressed in various tissues including the developing brain retina and liver. Notably Lhx2 plays a role in embryonic development by impacting the growth and differentiation of several tissue types.
Biological function summary
Researchers link the Lhx2 protein to key processes in cellular differentiation and organ development. Lhx2 often partners within transcriptional complexes that guide cellular fate decisions. By interacting with other cofactors Lhx2 influences the expression of genes involved in tissue-specific differentiation and morphogenesis. This regulatory action is essential for the proper formation and function of certain organs and structures in the body.
Pathways
Lhx2 impacts several intricate biological pathways notably in neural development. It functions within the Wnt signaling pathway where it modulates neural progenitor cell populations in the central nervous system. Through this pathway Lhx2 associates with proteins like β-catenin to control gene expression linked to brain development. Additionally Lhx2 is involved in the Sonic hedgehog (Shh) signaling pathway aligning with proteins such as Gli1 to influence neuronal patterning.
Mutations or dysregulation of Lhx2 have connections to conditions like anophthalmia and brain malformations. Aberrant expression of Lhx2 can disrupt normal development contributing to these congenital anomalies. Furthermore Lhx2's interaction with proteins including Pax6 plays a role in ocular development and anomalies in these interactions can lead to abnormal eye formation. Researchers continue to investigate the significance of Lhx2 in various developmental diseases to develop targeted therapeutic approaches.