Abcam, ab236277, Anti-Ku80 antibody

Size: 100µg / 100µL
Rabbit Polyclonal Ku80 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human XRCC5 aa 250-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human XRCC5 aa 250-500. The exact immunogen used to generate this antibody is proprietary information.P13010

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Ku80 protein also known as Ku p70 Ku antigen p70 and by gene identifiers such as Ku80 hum39 and 5c5 functions critically in DNA repair processes. With a molecular mass of approximately 80 kDa Ku80 is ubiquitously expressed in mammalian cells. It is an important component of the Ku heterodimer working with its partner Ku70 to detect and bind DNA double-strand breaks (DSBs). This action initiates repair by aligning with DNA ends to prevent break processing and sustain genome integrity.
Biological function summary
The Ku80 protein executes an important role in maintaining cellular stability by participating in the non-homologous end joining (NHEJ) pathway. Ku80 forms a complex with DNA-PKcs (DNA-dependent protein kinase catalytic subunit) which activates the kinase to phosphorylate itself and other proteins facilitating the repair of DSBs. Its expression is significant in tissues that undergo high rates of cellular turnover and are experienced with DNA damage where the DNA repair mechanisms are important for regular cell cycle progression.
Pathways
DNA repair and V(D)J recombination are principal domains where Ku80 shows its influence. In the context of DNA repair Ku80's partnership with DNA-PKcs as part of the NHEJ repair pathway is fundamental in fixing DNA damage from environmental insults or normal cellular metabolism. In the immune development process through V(D)J recombination Ku80 partners with other proteins like the Artemis complex to perform programmed DNA rearrangements required for the diversity of immune receptors.
Ku80 has a significant relation to cancer and immune deficiencies. Defects in Ku80 function can lead to increased sensitivity to ionizing radiation and a higher propensity for cancer development because of impaired DNA repair capabilities. Moreover in immune-related disorders inadequate Ku80 performance affects the maturation of lymphocytes leading to immune system malfunction. Its malfunctioning connection with proteins like DNA-PKcs and Artemis highlights its critical roles in both tumor suppression and immune system competence.