Abcam, ab236612, Anti-CYB5B antibody

Size: 100µL
Rabbit Polyclonal CYB5B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5B aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CYB5B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O43169

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CYB5B protein also known as Cytochrome b5 type B is a microsomal enzyme with an approximate mass of 13.4 kDa. It resides mainly in the endoplasmic reticulum membrane playing a role in electron transfer activities. This protein is expressed in various tissues with notable presence in the liver and adrenal glands. Unlike its mitochondrial counterparts CYB5B is involved in several mixed-function oxidase systems contributing to the catalysis of reactions through electron transfer.
Biological function summary
Cytochrome b5 type B interacts with a wide range of oxidoreductase enzymes which assists in lipid biosynthesis. It acts as a component of the electron transport chain within the endoplasmic reticulum supporting the desaturation and elongation of fatty acids. Though not part of a larger complex its role in electron donation is critical for maintaining cellular lipid homeostasis and synthesizing essential fatty acids.
Pathways
CYB5B plays a role in both the fatty acid metabolic process and the sterol biosynthetic pathway. It provides electrons to desaturase enzymes like stearoyl-CoA desaturase facilitating the transformation of saturated fatty acids into unsaturated fatty acids. This electron relay is also integral to the modulation of cholesterol levels within cells and relates to proteins such as NADH-cytochrome b5 reductase that help transfer electrons from NADH to cytochrome b5.
CYB5B alterations can impact conditions such as fatty liver disease and adrenal hyperplasia. Its functional collaboration with enzymes such as cytochrome P450 isoforms can influence steroid hormone biosynthesis especially in adrenal tissues. The dysregulation of CYB5B activity might affect lipid metabolic stability making it a point of interest in understanding lipid-associated metabolic and endocrine disorders.