Abcam, ab236783, Anti-NANS antibody

Size: 100µL
Rabbit Polyclonal NANS antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NANS aa 100-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NANS aa 100-300. The exact immunogen used to generate this antibody is proprietary information.Q9NR45

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N-acetylneuraminate synthase commonly known as NANS catalyzes the formation of N-acetylneuraminic acid (Neu5Ac) from N-acetyl-D-mannosamine 6-phosphate and phosphoenolpyruvate. This process is important in the sialic acid biosynthesis pathway. NANS has a molecular weight of approximately 46 kDa and is expressed in various tissues most abundantly in the liver and brain. These tissues show significant metabolic activity suggesting the enzyme's active involvement in cellular processes.
Biological function summary
This enzyme plays a significant role in the synthesis of sialic acids which are essential components of glycoconjugates such as glycoproteins and glycolipids. NANS is not known to associate with any specific large protein complexes but its action is necessary for generating substrates that contribute to protein glycosylation and the modification of lipids. These modifications influence cell-cell communication neurite outgrowth and immune response modulation.
Pathways
This enzyme is pivotal in the sialic acid biosynthesis pathway impacting the production of glycoproteins and glycolipids. In this context NANS interacts with key enzymes like sialyltransferases which further transfer sialic acids to the proteins and lipids. This pathway is essential in glycobiology affecting cellular interactions and signal transduction processes.
Mutations in the NANS gene have been linked to developmental delay syndromes especially NANS deficiency. These symptoms appear due to disrupted sialic acid production leading to abnormal glycosylation patterns. Furthermore the alteration in NANS activity indirectly affects proteins such as integrins and selectins contributing to immune deficiencies and metabolic syndromes.