Abcam, ab236895, Human IFN gamma High Sensitivity ELISA Kit

Size: 1 x 96Tests
Human IFN gamma High Sensitivity ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IFN gamma with a sensitivity of 2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2 pg/mL,
Range:12.5 - 1600 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IFN gamma High Sensitivity ELISA Kit ab236895 is a rapid single-wash 90-min sandwich ELISA kit to measure Human IFN gamma in Serum, Citrate Plasma, EDTA Plasma, Saliva, and Cell culture supernatant. This Simplestep sensitivity is 2 pg/mL.
How the assay works
Human IFN gamma SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
This immunoassay is calibrated against a highly purified human IFN gamma. The NIBSC/WHO unclassified purified human IFN gamma preparation 87/586 was evaluated in this kit.
The dose response curve of the unclassified standard IFN gamma parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human IFN gamma kit to approximate NIBSC 87/586 units, use the equation below.
NIBSC (87/586) approximate value (IU/mL) = 0.01113 x SimpleStep Human IFN gamma value (pg/mL).
Human IFN gamma High Sensitivity ELISA Kit ab236895 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
How other researchers are using Human IFN gamma High Sensitivity ELISA Kit ab236895
Human IFN gamma High Sensitivity ELISA Kit ab236895 has been used to bispecific antibodies as potential cancer therapeutics
References:
X. Pang, et.al., 2022, PMID: 35317514
Related and Recommended products
Human IFN gamma High Sensitivity ELISA Kit ab236895 is commonly used to measure IFN gamma as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA Kit
ab181421
- Human IL-6 ELISA Kit
ab178013
- Human IL-1 beta ELISA Kit
ab214025
To measure IFN gamma in our 384-well format:
- Human Interferon gamma ELISA Kit
ab300323

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.
Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.