Abcam, ab237545, Anti-PFKM antibody

Size: 100µg / 100µL
Rabbit Polyclonal PFKM antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKM aa 150-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PFKM aa 150-350. The exact immunogen used to generate this antibody is proprietary information.P08237

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PFKM also known as phosphofructokinase muscle type is a protein involved in glycolysis. It functions as an enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate. This reaction is important for the regulation of glucose metabolism. The PFKM protein has an approximate mass of 85 kDa and is mainly expressed in muscle tissues. Alternate names for PFKM include 6-phosphofructokinase type M and PFK-1 highlighting its critical role in glycolytic processes.
Biological function summary
PFKM acts as an important regulatory component in glycolysis because it controls the rate-limiting step of this metabolic pathway. It forms a homo-tetramer complex which can vary in its exact subunit composition to modulate activity based on cellular energy requirements. The presence of allosteric sites on PFKM allows it to respond to different metabolic signals such as ATP and AMP levels highlighting its role in energy homeostasis. The ability to alter its conformation and enzymatic activity enables PFKM to efficiently regulate energy production and consumption at the cellular level.
Pathways
PFKM plays a pivotal role in the glycolytic pathway facilitating the breakdown of glucose for energy production. This enzyme not only relates to the glycolysis pathway but also interacts with other key proteins such as hexokinase and pyruvate kinase forming a tightly controlled network for efficient metabolic flow. PFKM's activity influences downstream processes including the citric acid cycle and oxidative phosphorylation through its provision of pyruvate.
Defects in PFKM are associated with glycogen storage disease type VII also known as Tarui's disease. This disorder results from a deficiency in PFKM activity leading to the accumulation of glycogen in muscle tissues and resulting in exercise intolerance. Additionally PFKM dysfunction has connections to cancer given that many cancer cells exhibit increased glycolytic activity. Considering its role in disease studying PFKM can provide insights into potential therapeutic targets for conditions characterized by metabolic abnormalities.