Abcam, ab237560, Recombinant Salmonella typhimurium PrgI protein (His tag)

Size: 100µg
Recombinant Salmonella typhimurium PrgI protein (His tag) is a Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Full Length protein, in the 1 to 80 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P41784,
Animal free:No,
Carrier free:No,
Species:Salmonella enterica subsp. enterica serovar Typhimurium str. LT2,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PrgI also known as PrgI protein serves as an essential component of the type III secretion system (T3SS) in certain bacteria notably Salmonella enterica. This protein functions as a needle component playing a mechanical role in piercing host cell membranes to deliver bacterial effectors directly into host cells. PrgI is a small protein with an estimated mass of around 9.6 kDa. Typically PrgI is expressed in pathogenic bacteria where T3SS is active which includes various specialized cells involved in infection.
Biological function summary
PrgI facilitates the translocation of virulence factors from the bacterial cytoplasm to host cell cytosol. PrgI forms a part of the needle complex a large structure spanning both bacterial membranes. The needle complex works together with other proteins such as PrgH and PrgK to ensure the successful injection of effector proteins into host cells. This protein assembly allows the bacterium to manipulate host cell functions aiding bacterial survival and replication within the host.
Pathways
PrgI integrates into the pathogenicity pathways of bacteria like Salmonella. It plays a significant role in the Salmonella pathogenicity island 1 (SPI-1) pathway which is important for enabling bacterial invasion into non-phagocytic cells. In this context PrgI works closely with other T3SS components such as InvG and SicA to mediate effector protein translocation and establish infection. Understanding PrgI's role highlights its importance in the formation and regulation of secretion systems that contribute to bacterial pathogenicity.
PrgI strongly connects to foodborne illnesses caused by Salmonella infections. Such infections can lead to gastroenteritis and more severe systemic diseases like typhoid fever. PrgI's interactions with proteins like SipA and SipC amplify its role in promoting bacterial virulence and disease progression. Research into PrgI not only aids in comprehending these bacterial infections but also provides opportunities to develop interventions that disrupt its function potentially reducing the disease burden stemming from Salmonella and related pathogens.