Abcam, ab237930, Anti-ZAP70 antibody [ZAP70/2035] - BSA and Azide free

Size: 100µg
Mouse Monoclonal ZAP70 antibody. Carrier free. Suitable for IHC-P, ICC, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZAP70 aa 200-400.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:ZAP70/2035,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, ICC, Protein ArraySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ZAP70 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.P43403

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from Bioreactor Concentrate by Protein A/G., Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZAP70 also known as Zeta-chain-associated protein kinase 70 is a tyrosine kinase that plays an important role in T-cell receptor signaling. The protein has a molecular mass of approximately 70 kDa. ZAP70 is mainly expressed in T cells and natural killer (NK) cells. It gets activated upon T-cell receptor engagement initiating downstream signaling cascades essential for T-cell function and adaptive immune response.
Biological function summary
ZAP70 is instrumental in the signaling cascade that activates T cells in response to antigen recognition. It forms part of a larger signaling complex following the engagement of the T-cell receptor with antigens. This complex transmits critical activation signals leading to further cellular responses such as cytokine production and cell proliferation. ZAP70's function ensures appropriate immune responses and helps maintain immune system balance.
Pathways
ZAP70 plays an integral role in T-cell receptor signaling pathways. This protein interacts with other signaling molecules like Lck and LAT facilitating the transmission of activation signals within the cell. As part of the T-cell activation pathway ZAP70 helps to bridge receptor-ligand interactions with cellular responses enabling the immune cells to combat infections effectively.
ZAP70 mutations or dysregulations can lead to severe immunodeficiencies or autoimmune diseases. For example ZAP70 deficiency results in severe combined immunodeficiency (SCID) which severely impairs immune function. Additionally aberrant ZAP70 activity has links to certain autoimmune disorders like rheumatoid arthritis where it influences abnormal immune signaling. Understanding the biochemical pathways that involve ZAP70 provides insight into managing these diseases and developing therapeutic strategies.