Abcam, ab23875, Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody

Size: 50µL
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab23875) is a rabbit polyclonal antibody detecting AMPK alpha 1 in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - Over 70 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRKAA1 phospho T183. The exact immunogen used to generate this antibody is proprietary information.Q13131,
Specificity:ab23875 recognises the phosphorylated forms of AMPK alpha 1 (T183) and AMPK alpha 2 (T172).

Product details:
What is this antibody validated in?
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab23875) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of AMPK alpha 1?
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) (ab23875) specifically detects a band for AMPK alpha 1 (UniProt: Q13131) at a molecular weight of 62kDa.
Trusted by the scientific community
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) (ab23875) was first used in a scientific publication in 2005 and has been cited over 70 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab23875 was purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated AMPK. The final product is generated by affinity chromatography using a AMPK derived peptide that is phosphorylated at threonine 172., Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot