Abcam, ab238944, Anti-AKT1 + AKT2 antibody [EPR18405] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AKT1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18405,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab238944 is the carrier-free version of
ab188099
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The AKT1 and AKT2 proteins also known as Protein Kinase B (PKB) isoforms are serine/threonine kinases with molecular masses approximately 56 kDa each. These proteins play mechanical roles as critical regulatory components in cell signaling pathways. AKT1 and AKT2 get activated via phosphorylation events particularly at threonine 308 and serine 473 for AKT1 facilitating downstream effects. AKT1 and AKT2 are expressed ubiquitously across diverse tissue types including heart brain lung and other vital organs. Their cellular presence ensures robust signaling responses across different physiological contexts.
Biological function summary
AKT1 and AKT2 regulate key cellular processes such as glucose metabolism apoptosis and cell proliferation. The two proteins act as part of the complex involving phosphoinositide 3-kinase (PI3K) affecting the survival and growth of cells. Through their actions these kinases impact various cellular mechanisms maintaining homeostasis and cellular integrity under stress. Their ability to modulate such essential processes illustrates their significance in maintaining cellular life and function.
Pathways
AKT1 and AKT2 are integral components of the PI3K/AKT/mTOR signaling pathway a pathway relevant in the regulation of cell growth and survival. Within this pathway AKT1 and AKT2 interact closely with proteins such as PI3K and mTOR executing signaling cascades essential for normal cellular functions. Additionally they exhibit connections to the insulin signaling pathway where they interact with insulin receptor substrates (IRSs) influencing glucose uptake and energy balance within the cell.
AKT1 and AKT2 have substantial relevance to several pathological conditions such as cancer and type 2 diabetes. Aberrant expression or mutations in AKT1 and AKT2 can lead to uncontrolled cell proliferation contributing to oncogenesis. Likewise these proteins affect insulin signaling and dysregulation can result in insulin resistance and type 2 diabetes. During these disease states AKT1 and AKT2 show considerable interactions with proteins like PTEN a phosphatase that negatively regulates the PI3K/AKT pathway highlighting their roles in pathophysiological conditions.