Abcam, ab238949, Anti-Claudin 1 antibody [EPRR18871] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Claudin 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Recombinant fragment samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPRR18871,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, IP, Flow Cyt (Intra), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab238949 is the carrier-free version of
ab211737
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Claudin 1 also known as CLDN1 is a protein that plays a fundamental role in the formation of tight junctions in the epithelial cells. This claudin protein has a molecular weight of approximately 22 kDa. You can find it expressed in various tissues including the skin liver kidney and lungs. Claudin 1 belongs to the claudin family which consists of transmembrane proteins involved in sealing the space between adjacent cells therefore ensuring tissue integrity.
Biological function summary
This claudin protein contributes to establishing and maintaining the barrier function that controls paracellular transport which is the movement of substances between cells. Claudin 1 participates as a critical component of the tight junction complex and it interacts with other proteins such as occludin and zonula occludens. These interactions contribute to the regulation and maintenance of cell polarity and signaling.
Pathways
Claudin 1 is involved in the epithelial cell signaling pathways that influence cellular proliferation and differentiation. One significant pathway is the Wnt signaling pathway where claudin 1 cooperates with other claudins and proteins like β-catenin to regulate gene transcription. Additionally it is implicated in the MAPK pathway which involves signaling cascades that affect cell growth division and response to external stress.
Claudin 1 shows a strong association with cancers particularly hepatocellular carcinoma and melanoma. In these conditions changes in claudin 1 expression levels can affect tumor progression and metastasis. It also associates with other proteins like E-cadherin impacting cell adhesion and epithelial-mesenchymal transition in cancerous cells. Moreover abnormalities in claudin 1 expression relate to skin disorders such as ichthyosis which is marked by a dysfunction in the skin barrier.