Abcam, ab239015, Anti-BTF3 antibody [EPR16495] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal BTF3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16495,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239015 is the carrier-free version of
ab203517
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BTF3 also known as Basic Transcription Factor 3 is an important transcription factor weighing around 21 kDa. It plays a mechanical role in initiating and regulating transcription by binding to RNA polymerase II facilitating gene expression. BTF3 is ubiquitously expressed in various tissues serving as an essential component in cellular processes. Its activity is not confined to specific cell types making it an important element across the organism.
Biological function summary
BTF3 contributes significantly to the regulation of transcription and is part of the transcriptional preinitiation complex. This complex is necessary for the transcription of class II genes influencing cell cycle and growth. Through its interaction within this complex BTF3 assists in recruiting RNA polymerase II to specific gene promoters. It plays a role in maintaining gene expression stability affecting how cells respond to different stimuli.
Pathways
BTF3 is integrally involved in the transcriptional regulation pathways. It directly participates in the transcription initiation process working with proteins such as TFIIB and TFIID within the transcriptional machinery. This interaction places BTF3 as a critical link in the gene expression regulatory network impacting the overall cellular response to environmental and physiological signals.
BTF3 has associations with several conditions including cancer and neurodegenerative diseases. In cancer the overexpression of BTF3 leads to unchecked cell proliferation linking it with tumorigenesis. It interacts with proteins such as MYC which is vital in cell cycle regulation and apoptosis. In neurodegenerative conditions the disruption of BTF3's normal function may contribute to pathological progress showing its broad relevance in both health and disease.