Abcam, ab239522, Anti-ASPA antibody [EPR22072] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ASPA antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22072,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IP, IHC-Fr, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239522 is the carrier-free version of
ab223269
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aspartoacylase also known by its abbreviation ASPA is an enzyme that breaks down N-acetyl-L-aspartate into aspartate and acetate. It has a mass of about 60 kDa. ASPA is highly expressed in the brain especially in oligodendrocytes which are a type of central nervous system cell responsible for producing the myelin sheath that surrounds nerve cells. Its enzymatic activity is dependent on divalent metal ions which are required for its function.
Biological function summary
ASPA plays an important role in myelin sheath maintenance and acetate metabolism. This protein doesn't form part of a complex but acts independently in the cytoplasm and peroxisomes of cells. Its function is significant for the provision of acetate for lipid synthesis which is necessary for myelin production. By regulating N-acetyl-L-aspartate levels in the brain ASPA activity is linked directly to protecting nerve cells' structural integrity.
Pathways
The ASPA enzyme is involved in the aspartate and acetyl-CoA pathways which are central to cellular energy balance and lipid metabolism. Its enzymatic role facilitates the recycling of acetate which is vital to the synthesis of myelin lipids. The relationship of ASPA with enzymes like acetyl-CoA synthetase highlights its involvement in broader biochemical pathways fundamental to cell metabolism and growth.
Deficiencies in ASPA are linked to Canavan disease a rare and inherited neurodegenerative condition characterized by white matter sponginess and loss of motor skills. This disorder results from mutations in the ASPA gene leading to dysfunctional enzyme activity. Additionally changes in ASPA expression levels might contribute to demyelinating disorders where proteins like myelin basic protein are also often involved making ASPA an important target for studies exploring nervous system diseases.