Abcam, ab239539, Recombinant Influenza A Polymerase acidic protein (His tag)

Size: 100µg
Recombinant Influenza A Polymerase acidic protein (His tag) is a Influenza A virus (A/X-31(H3N2)) Fragment protein, in the 124 to 247 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9IQ47,
Animal free:No,
Carrier free:No,
Species:Influenza A virus (A/X-31(H3N2)),
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Polymerase acidic protein also known as PA plays an important role in the transcription and replication of the influenza virus RNA genome. PA partners with PB1 and PB2 to form the influenza virus RNA polymerase complex. This heterotrimeric complex allows the virus to hijack host cell mechanisms to replicate. The polymerase acidic protein has a mass of about 83 kDa and gets expressed within nuclear compartments of infected host cells.
Biological function summary
The polymerase acidic protein drives mRNA synthesis by cleaving host pre-mRNA and uses this as a primer for viral transcription a process often referred to as "cap-snatching". PA collaborates closely with PB1 and PB2 forming the polymerase complex that stabilizes the influenza virus's ability to propagate. This collaboration allows the virus to efficiently use host resources for synthesizing its necessary proteins and genomic RNA.
Pathways
Many research studies show that polymerase acidic protein plays a significant role in the virus's life cycle pathways. It significantly interacts with the host's transcriptional machinery during the viral replication cycle. The respiratory infection pathway where influenza virus operates depends heavily on interactions between PA PB1 and PB2 as well as between these viral proteins and host factors that facilitate viral proliferation.
Research associates polymerase acidic protein with the pathology of influenza a highly contagious respiratory illness. The impact of PA on disease progression is related to its role in viral replication. Connections between PA and other viral proteins such as PB1 and PB2 further contribute to the virus's ability to evade immune response and cause persistent infections. Understanding PA's function and interactions can help develop antiviral strategies and influenza therapeutics.