Abcam, ab239542, Recombinant Pectate lyase 1 protein (Tagged)

Size: 100µg
Recombinant Pectate lyase 1 protein (Tagged) is a Hesperocyparis arizonica Full Length protein, in the 22 to 367 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9SCG9,
Animal free:No,
Carrier free:No,
Species:Hesperocyparis arizonica,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pectate lyase 1 also known as PL1 or PecL1 is an enzyme that catalyzes the cleavage of pectate a form of pectin into smaller units through a lyase mechanism. The enzyme exhibits a molecular mass of approximately 40 kDa. Pectate lyase 1 is expressed in various plant tissues particularly in areas undergoing cell wall modification such as elongating and ripening fruits leaf abscission zones and germinating seeds. The presence of this enzyme is significant in the breakdown of pectin found in the plant cell wall matrix.
Biological function summary
The action of Pectate lyase 1 aids in cell wall degradation and softening making it essential in the plant's natural growth and development processes. During processes such as fruit ripening leaf fall and seed germination Pectate lyase 1 loosens the cell walls by breaking down pectin which is a significant component of plant cell walls. Pectate lyase 1 operates in coordination with other cell wall-degrading enzymes such as polygalacturonase within a complex that works synergistically to facilitate plant tissue differentiation and development.
Pathways
The activity of Pectate lyase 1 fits into the cell wall modification and glycosaminoglycan degradation pathways. In the cell wall modification pathway Pectate lyase 1 interacts with proteins like polygalacturonase to dismantle pectate contributing to cell separation and expansion. Additionally the enzyme participates in the glycosaminoglycan degradation pathway highlighting its involvement in polysaccharide breakdown alongside other enzymes ensuring the dynamic nature of plant cell walls to accommodate growth and environmental adaptation.
Disruptions in the expression or activity of Pectate lyase 1 can impact both agricultural and environmental contexts. One such impact is fruit softening where overactivity of this enzyme leads to excessive softening and post-harvest losses. Another disease associated with Pectate lyase 1 is bacterial soft rot where pathogens exploit the enzymatic action of Pectate lyase 1 to invade plant tissues. In these contexts the role of other associated proteins such as cellulase becomes important as they work in tandem with Pectate lyase 1 to accelerate tissue degradation during plant pathogenesis.