Abcam, ab23990, Anti-MHC Class II antibody [MRC OX-6]

Size: 100µg
Anti-MHC Class II antibody [MRC OX-6] (ab23990) is a mouse monoclonal antibody detecting MHC Class II in IHC-P, ICC/IF . Suitable for Mouse, Rat . - Over 50 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MRC OX-6,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse recommendation is based on the ICC results. We do not guarantee IHC-P for mouse.

Product details:
What is this antibody validated in?
Anti-MHC Class II antibody [MRC OX-6] (ab23990) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse, Rat samples.
Trusted by the scientific community
Anti-MHC Class II [MRC OX-6] (ab23990) was first used in a scientific publication in 2005 and has been cited over 50 times in peer-reviewed journals.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MHC Class II also known as MHC II or human leukocyte antigen (HLA) class II is a protein complex with a molecular mass generally around 55 kDa. This protein is mainly expressed on the surface of antigen-presenting cells such as dendritic cells macrophages and B cells. It plays an important role in the immune system by presenting extracellular antigens to T helper cells. MHC II molecules are composed of two polypeptide chains alpha and beta which associate to form a heterodimer on the cell surface.
Biological function summary
MHC Class II molecules facilitate the immune system's recognition and response to pathogens by presenting processed antigenic peptides to CD4+ T lymphocytes. This activity involves their assembly within the endosomal-lysosomal system where they bind peptides derived from endocytosed proteins. MHC II is part of the major histocompatibility complex which includes other related molecules such as MHC Class I (HLA class I). The binding and presentation of peptides are central for the coordination of adaptive immune responses enhancing pathogen clearance and maintaining immunological memory.
Pathways
MHC Class II operates within the antigen processing and presentation pathway important for adaptive immunity. Its interaction with CD4+ T cells stimulates downstream signaling pathways leading to T cell activation and differentiation. Related proteins in the antigen presentation pathway include invariant chain (Ii) which guides MHC II to the endosome and HLA-DM which facilitates peptide loading. Together these proteins and pathways work to ensure effective immune surveillance and response.
Alterations in MHC Class II expression or function are associated with autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. In these conditions improper antigen presentation may lead to an inappropriate immune response against self-proteins. The involvement of MHC II in disease is often linked to specific HLA alleles which vary among individuals and influence susceptibility to autoimmune disorders. Additionally the abnormal function of connected proteins like cytokines from activated T helper cells can exacerbate these immune conditions.