Abcam, ab239934, Anti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal EN1 / Engrailed 1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3282,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:We have data to suggest no cross-reactivity with EN2/Engrailed 2, please contact us if you require further details/experimental data

Product details:
ab239934 is the carrier-free version of
ab108598
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target EN1 also known as Engrailed 1 is a homeobox-containing transcription factor involved in developmental processes. EN1 protein weighs approximately 40 kDa and is expressed primarily in the developing brain especially in the midbrain and hindbrain regions during embryonic development. It also appears in the skeletal and nervous systems where it regulates specific gene expression patterns. The protein sometimes referred to as EN1 protein plays a significant role in tissue specification and morphogenesis during early developmental stages.
Biological function summary
The Engrailed 1 protein influences pattern formation by controlling the transcription of key developmental genes. Part of several transcriptional complexes it binds to DNA sequences aiding in the specification of positional identities during embryogenesis. EN1 also has significant functions in the maintenance of cell fates and is essential for the differentiation of numerous cell types. Through these processes EN1 ensures proper segmental development and organogenesis.
Pathways
Engrailed 1 integrates into multiple signaling pathways that control cell development including the Wnt and Hedgehog pathways. Within the Wnt pathway EN1 acts alongside proteins like β-catenin to manage cellular responses vital for neuronal and skeletal formation. It also interacts with other transcription factors such as Pax2 in the regulation of pathways linked to neural development.
EN1 has connections to conditions like Parkinson's disease and certain congenital disorders like cerebellar hypoplasia. The dysregulation or mutation of EN1 influences the onset and progression of these diseases. It relates to the serotoninergic and dopaminergic systems reflecting its importance in maintaining normal neurotransmitter functions. In Parkinson's the interaction of EN1 with proteins such as α-synuclein demonstrates its potential impact on neurodegeneration and neuron survival.