Abcam, ab239939, Anti-TRF2 antibody [EPR3517(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TRF2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3517(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, Flow Cyt (Intra), WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239939 is the carrier-free version of
ab108997
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRF2 also known as Telomeric Repeat-binding Factor 2 is a protein important for telomere protection and maintenance. TRF2 weighs approximately 55 kDa and is widely expressed in human tissues especially in those with high turnover rates. This protein binds specifically to double-stranded telomeric DNA and forms a protective cap at the end of chromosomes helping to regulate telomere length and integrity. By securing the telomere's structure TRF2 prevents chromosomes from being recognized as damaged DNA.
Biological function summary
TRF2 plays a central role in telomere function by assembling into the shelterin complex. This complex which includes other proteins like TRF1 TIN2 POT1 TPP1 and RAP1 is essential for controlling telomere length and stability. TRF2 is responsible for inhibiting DNA damage response pathways at telomeres ensuring that the natural chromosomal ends are not mistaken for double-strand breaks. This suppression of unwanted DNA damage response is important for normal cell division and avoiding cellular senescence.
Pathways
Researchers have placed TRF2 within the DNA repair and telomere maintenance pathways. TRF2 interacts with several other proteins such as Ku70/80 and RAD51 that are instrumental in the DNA repair process. Furthermore TRF2 influences the Alternative Lengthening of Telomeres (ALT) pathway which is a telomere lengthening process found in some cancer cells. These connections highlight TRF2's role in maintaining genomic stability through its capacity to regulate telomere-associated proteins and pathways.
TRF2 has been linked to cancer and aging. Elevated levels of TRF2 have been detected in various cancers including breast and prostate cancer suggesting a role in tumorigenesis where it contributes to the stability of cancer cell telomeres. Additionally defects in TRF2 or its interaction with partner proteins like POT1 can lead to dysfunctional telomeres which contribute to the aging process and age-related diseases by affecting cell proliferation and survival. These links demonstrate the importance of TRF2 in normal cellular function and its potential as a therapeutic target in disease intervention.