Abcam, ab240056, Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TPM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5159,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240056 is the carrier-free version of
ab133292
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tropomyosin 1 (alpha) and Tropomyosin 3 are actin-binding proteins with key roles in stabilizing actin filaments within muscle and non-muscle cells. Often referred to as TPM1 and TPM3 these proteins have molecular masses of approximately 32 kDa and 33 kDa respectively. TPM1 and TPM3 are expressed in various tissues such as heart skeletal muscle and brain. They help maintain structural integrity of the cytoskeleton by interacting with actin filaments providing essential support for cellular functions.
Biological function summary
Tropomyosin isoforms bind along the length of actin filaments regulating their interaction with myosin and other actin-binding proteins. TPM1 and TPM3 form part of a complex that includes troponin contributing to the calcium-dependent regulation of muscle contraction. In non-muscle cells they regulate cell shape cytokinesis and intracellular transport. Through their role in stabilizing actin filaments these proteins influence many cellular processes.
Pathways
TPM1 and TPM3 are essential for muscle contraction and cytoskeletal dynamics. They play significant roles in the actin cytoskeleton and cardiac muscle contraction pathways. Tropomyosins interact with proteins like myosin and troponin to modulate muscle contraction. They ensure effective actin-myosin interaction by allowing proper binding and release cycles during contraction and relaxation.
TPM1 and TPM3 mutations have associations with conditions such as hypertrophic cardiomyopathy and nemaline myopathy. These genetic mutations can alter their ability to regulate actin filament function contributing to abnormal contraction and muscle weakness. In hypertrophic cardiomyopathy TPM1 mutations often occur alongside mutations in proteins like myosin heavy chain and cardiac myosin-binding protein C compounding the disease's effects and presenting critical challenges in cardiac function.