Abcam, ab240113, Anti-ME2 antibody [EP7217] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ME2 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP7217,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240113 is the carrier-free version of
ab139686
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein ME2 also known as mitochondrial malic enzyme 2 is a mitochondrial enzyme with a molecular mass of approximately 65 kDa. It catalyzes the oxidative decarboxylation of malate to pyruvate in the tricarboxylic acid cycle converting NAD+ to NADH. ME2 is primarily expressed in tissues with high metabolic activity such as liver and muscle. The enzyme plays a role in energy production and is found across various species reflecting its evolutionary conservation.
Biological function summary
ME2 is an important regulator of cellular metabolism and part of the mitochondrial malate-aspartate shuttle. The enzyme helps facilitate the transfer of reducing equivalents across the mitochondrial membrane. ME2 does not function alone and might interact with other metabolite transporters in mitochondrial membranes contributing to the balance of metabolic intermediates and maintenance of cellular energy homeostasis.
Pathways
Malate metabolism and energy production involve ME2's role in the tricarboxylic acid cycle and glycolysis. The tricarboxylic acid cycle requires ME2 for effective conversion of nutrients into usable energy. ME2 links with other metabolic enzymes including citrate synthase for efficient energy flow. Additionally the enzyme's activity affects the synthesis of biosynthetic intermediates integrating nutrient signals with bioenergetic demands.
ME2 alterations can impact conditions like metabolic syndromes and neurodegenerative diseases. Research indicates ME2 changes might relate to diabetes where altered energy metabolism disrupts insulin signaling. ME2 also bears connection to Alzheimer's disease likely due to its involvement in mitochondrial dysfunction a characteristic of the disorder. Understanding these links can guide therapeutic strategies aimed at correcting metabolic disturbances.