Abcam, ab240143, Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal OPA1 antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11057(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Recent lab testing showed that the antibody detects the band of interest at the proper molecular weight in several cell and tissue lysates. However, the antibody also detects a band at about 50kDa. We could not find in the literature any references on what this band could be. On our test, the 50kDa band detections decreased by decreasing the antibody concentration.

Product details:
ab240143 is the carrier-free version of
ab157457
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OPA1 also known as optic atrophy 1 is a dynamin-related GTPase protein important for mitochondrial fusion. OPA1 has a molecular weight of about 120 kDa and is present mostly in the inner mitochondrial membrane. It promotes the maintenance of mitochondrial DNA cristae structures and the modulation of mitochondrial dynamics. Expression of OPA1 occurs in tissues with high energy demands including the retina brain and muscles. Detection of the OPA1 protein can be done using techniques such as Western blot and it reveals different isoforms generated through alternative splicing.
Biological function summary
Mitochondrial dynamics involving OPA1 ensure energy production efficiency and cell health. OPA1 plays a role in mitochondrial fusion by forming a complex with mitofusins MFN1 and MFN2. This complex maintains the integrity of mitochondrial networks facilitates proper respiratory function and prevents apoptosis by regulating cristae junctions. It also participates in the stress response particularly in the preservation of the mitochondrial structure and function under challenging conditions.
Pathways
OPA1 integrates into the mitochondrial fusion and fission pathways important for cellular energy metabolism. It works alongside proteins like DRP1 in balancing these processes. The involvement in these pathways is essential for cellular adaptation to metabolic needs and stress. OPA1 also has a relationship with the PINK1/Parkin pathway where its regulation affects mitophagy a process of clearing damaged mitochondria. These interactions highlight the importance of OPA1 in maintaining cellular and mitochondrial homeostasis.
Mutations in OPA1 have been linked to autosomal dominant optic atrophy and a range of neurodegenerative conditions. The protein’s dysfunction leads to the degeneration of the retinal ganglion cells and their axons resulting in vision loss. OPA1 also shows connections to disorders like Charcot-Marie-Tooth disease where its interaction with other proteins like MFN2 plays a role. Deficiency or dysfunction of OPA1 disrupts mitochondrial dynamics leading to cellular energy deficits and contributing to disease pathophysiology.