Abcam, ab240158, Anti-NELFe antibody [EPR11600] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NELFE antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11600,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240158 is the carrier-free version of
ab170104
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NELFe also known as Negative Elongation Factor E or NELF-A plays an important role in regulating transcription elongation. NELFe has a mass of approximately 66 kDa. Expression of NELFe occurs broadly across various tissues which suggests its involvement in numerous cellular processes. This protein forms part of the NELF complex which interacts with RNA Polymerase II pausing transcription at specific genes. NELFe along with other components contributes to the binding to nascent RNA and halting transcription temporarily.
Biological function summary
NELFe influences gene expression by controlling the pausing of RNA Polymerase II which impacts the timing and localization of transcription. NELFe operates within a larger complex known as the Negative Elongation Factor complex that consists of NELF-A (NELFe) NELF-B NELF-C/D and NELF-E. This complex enables precise regulation of transcriptional pausing and subsequent release when appropriate signals arrive. The coordinated action of this complex ensures proper gene expression profiles necessary for development and cell differentiation.
Pathways
NELFe fits prominently in the transcription regulation pathway where it controls RNA Polymerase II pausing and release. NELFe interacts with the protein P-TEFb (Positive Transcription Elongation Factor b) which phosphorylates NELF complex components to release the pause allowing elongation. Another significant pathway involves NELFe in the regulation of heat shock genes where the heat shock factor 1 (HSF1) modulates the transcriptional pause release in response to stress showing NELFe's role in cellular stress responses.
NELFe has links to cancer and viral infections where its dysregulation can affect normal gene expression. In certain cancers unregulated pausing or failure to release transcription elongation can contribute to uncontrolled cell proliferation. Furthermore in the context of human immunodeficiency virus (HIV) infections NELFe and its related NELF complex play a role in viral latency. The related protein Tat interacts with NELFe highlighting its importance in HIV transcription regulation. Understanding NELFe's role in these diseases provides insight into potential therapeutic targets for intervention.