Abcam, ab240233, Anti-beta Amyloid 1-42 antibody [EPR9296] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Amyloid-beta precursor protein antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9296,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240233 is the carrier-free version of
ab180956
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta Amyloid 1-42 often referred to as Abeta 42 or amyloid beta 1-42 is a peptide derived from the amyloid precursor protein (APP). This peptide consists of 42 amino acids and has a molecular weight of approximately 4.5 kDa. It is predominantly found in the brain especially within regions associated with memory and cognitive function. Beta amyloid 1-42 is a product of enzymatic cleavage by beta and gamma secretases from the larger APP molecule. Due to its aggregation-prone nature beta amyloid 1-42 is a focus in neuroscience research particularly concerning the formation of amyloid plaques.
Biological function summary
Amyloid beta 1-42 plays a central role in synaptic activity and neuronal health. While not inherently pathogenic its tendency to aggregate forms oligomers and fibrils which become toxic to neurons. This peptide contributes to synaptic dysfunction and neuronal cell death and is a major component of extracellular amyloid plaques found in brain tissue. Within the cellular environment amyloid beta 1-42 can interact with receptors membrane lipids and intracellular signaling mechanisms thereby affecting neuronal cell function.
Pathways
Amyloid beta 1-42 is important in the amyloidogenic pathway which is one of the critical components in APP processing. The pathway includes proteolytic enzymes like beta secretase (BACE1) and gamma secretase where misregulation may lead to excessive production or deposition of amyloid beta peptides. Another related pathway is the tau protein phosphorylation pathway which indirectly interacts with amyloid beta 1-42 due to shared signaling intermediates impacting tau stabilization and microtubule assembly in neurons.
Amyloid beta 1-42 plays a pivotal role in Alzheimer's disease a prevalent neurodegenerative disorder. The excessive accumulation of beta amyloid in the form of plaques is a hallmark of the disease contributing to cognitive decline. Also it has links to cerebral amyloid angiopathy where it deposits in the walls of blood vessels of the central nervous system. Both conditions may involve interactions with tau protein another pathological feature of Alzheimer's disease which undergoes hyperphosphorylation leading to neurofibrillary tangles formation.