Abcam, ab240322, Anti-PRMT3 antibody [EPR13279] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PRMT3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13279,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240322 is the carrier-free version of
ab191562
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein arginine methyltransferase 3 (PRMT3) plays an important role in the methylation of arginine residues on various substrates which influences protein-protein interactions signal transduction and gene expression. Also known as HRMT1L3 PRMT3 has an approximate molecular weight of 59 kDa. Researchers find this enzyme mainly in the cytoplasm although it can also localize in the nucleus. Expression of PRMT3 happens across multiple tissues with notable presence in the liver and lungs.
Biological function summary
PRMT3 methylates ribosomal protein S2 an activity that suggests its participation in ribosome assembly and nucleolar functions. It forms a complex with ribosomal proteins and several metabolic enzymes which highlight its involvement in protein synthesis and cellular metabolic regulation. The activity of PRMT3 regulates the biogenesis of ribosomes which are central to protein production in cells.
Pathways
PRMT3 influences various cellular processes like the ribosome biogenesis and the mTOR signaling pathway. Its interaction with the ribosome synthesis pathway aids in the maturation and assembly of ribosomal subunits. In the mTOR pathway PRMT3 relates to S6 kinase and eukaryotic initiation factors affecting cell growth and metabolism through the regulation of protein synthesis.
PRMT3 may influence the development of metabolic syndromes and certain cancers. Studies suggest a potential link between PRMT3 dysregulation and the onset of insulin resistance making it significant in the context of type 2 diabetes. Additionally abnormal PRMT3 expression connects with progression of malignancies potentially through interacting with oncogenic proteins and altering signaling pathways that regulate cell cycle and apoptosis.