Abcam, ab240647, Anti-HEXIM1 antibody [EPR23430-12] - ChIP Grade

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal HEXIM1 antibody. Suitable for IHC-P, IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23430-12,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, Flow Cyt (Intra), ICC/IF, IHC-P, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HEXIM1 also known as hexamethylene bis-acetamide inducible protein 1 is a protein with an approximate molecular mass of 49 kDa. It predominantly resides in the nucleus and is ubiquitously expressed across various tissues. This protein functions primarily in the regulation of transcription by modulating the activity of P-TEFb (Positive Transcription Elongation Factor b). HEXIM1 acts as an inhibitor by binding to the cyclin T1 and CDK9 components of P-TEFb preventing its role in promoting RNA polymerase II activity during transcription elongation.
Biological function summary
HEXIM1 serves a critical regulatory role within the transcription machinery in cells. It acts as an essential component of the HEXIM1/P-TEFb/7SK snRNP complex which helps maintain transcriptional homeostasis. This complex involves 7SK small nuclear RNA which makes the transcription process more efficient by sequestering P-TEFb in an inactive state. By doing this HEXIM1 controls the release and activation of P-TEFb ensuring accurate transcription regulation and preventing aberrant gene expression.
Pathways
HEXIM1 is an integral element in managing transcription elongation processes. It is known to be part of significant signaling pathways such as the P-TEFb dependent transcription pathway. HEXIM1 interacts with proteins like CDK9 and cyclin T1 that are fundamental to its regulatory function. By controlling these interactions HEXIM1 influences RNA polymerase II's transition into productive elongation mode essential for proper gene expression.
Scientists have associated dysregulation of HEXIM1 activity with several conditions including cancer and cardiac hypertrophy. Aberrant HEXIM1 function or expression can lead to unchecked cellular proliferation in cancer due in part to misregulated P-TEFb activity. Moreover disruptions in this protein's regulatory capability have links to cardiac disorders where related proteins like CDK9 play roles in pathological cardiac growth. Therefore HEXIM1 serves as a potential target for therapeutic interventions concerning transcriptional dysregulation-related diseases.