Abcam, ab240700, Anti-COX5B antibody

Size: 100µg
Rabbit Polyclonal COX5B antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Cytochrome c oxidase subunit 5B, mitochondrial aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Cytochrome c oxidase subunit 5B, mitochondrial aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P10606

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target COX5B also known as cytochrome c oxidase subunit 5B is a subunit of the cytochrome c oxidase (complex IV) in mitochondria. This protein has a mass of approximately 13.2 kDa and plays an essential role in the electron transport chain. COX5B aids in the terminal step of the mitochondrial respiratory chain by facilitating electron transfer from reduced cytochrome c to oxygen. This process occurs mainly in mitochondria-rich tissues such as muscles neurons and cardiac muscle where high energy demand exists.
Biological function summary
COX5B acts within the larger cytochrome c oxidase complex which consists of multiple subunits that work together to promote efficient oxidative phosphorylation. This subunit contributes to the enzymatic activity that pumps protons across the inner mitochondrial membrane a critical step for ATP synthesis. The operation of COX5B is important for cellular respiration and energy production impacting metabolic functions across various cell types. It maintains cellular energy homeostasis by regulating how efficiently cells convert nutrients into energy.
Pathways
COX5B plays an important role in the oxidative phosphorylation pathway and is a part of the electron transport chain (ETC). It interacts with other ETC proteins such as COX2 and COX3 to ensure the proper function of the chain. Furthermore COX5B's involvement in oxidative phosphorylation links it to pathways like ATP synthesis where it indirectly influences essential cellular processes such as apoptosis and cellular metabolism due to its role in energy production.
COX5B has associations with conditions involving mitochondrial dysfunction such as mitochondrial myopathy and mitochondrial encephalomyopathy. Alterations in COX5B expression or function might disrupt the electron transport chain compromising ATP production. These conditions often coexist with mutations or dysregulation in other mitochondrial proteins such as COX6A1 which indicate a broader spectrum of mitochondrial disorders. Understanding COX5B's role in such diseases could lead to insights into potential therapeutic targets for mitochondrial-related disorders.